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Western Blot analysis of 293 cells using CD6 Polyclonal Antibody.
Western Blot analysis of 293 cells using CD6 Polyclonal Antibody.
Western Blot analysis of 293 cells using CD6 Polyclonal Antibody.

CD6 Antibody

CSB-PA006156
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCD6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID923
  • Target name
    CD6
  • Target description
    CD6 molecule
  • Target synonyms
    CD6 antigen; T12; T-cell differentiation antigen CD6; TP120
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30203
  • Protein Name
    T-cell differentiation antigen CD6
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of T12/CD6 using anti-T12/CD6 antibody (A03913-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-T12/CD6 antigen affinity purified polyclonal antibody (Catalog # A03913-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for T12/CD6 at approximately 135 kDa. The expected band size for T12/CD6 is at 72 kDa.
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