Anti-CD70 [TAN1-6]

Absolute Antibody

Anti-CD70 [TAN1-6]

7

This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-

This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004; Taraban et al, 2006).

Flow Cytometry, ImmunoFluorescence, Western Blot, Neutralisation/Blocking

FC; WB; IF; blocking

Research Use Only

Monoclonal

TAN1-6

Cd70

CD70 antigen

CD27 ligand; Cd27l; CD27-L; CD27LG; CD70 antigen; Ki-24 antigen; Tnfs; Tnfsf7; Tnlg8a; tumor necrosis factor ligand 8a; tumor necrosis factor ligand superfamily member 7

Rabbit

IgG

CD70 antigen

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein

-20°C,2°C to 8°C

12352203