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ProductsBack/anti-CD74 (human), mAb (M-B741) (APC)
Binding of anti-CD74/APC to human Raji cells. Method: Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 microl of anti-CD74/APC at a 1:50 dilution (10 microg/ml). Cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.65 log10 fluorescent units when compared to Isoptype Control Mouse IgG2a/APC (#ANC-281-060). Binding was blocked when cells were pre-incubated 10 min with 20 microl of 0.5 mg/ml anti-CD30 antibody (#ANC-179-020).
Binding of anti-CD74/APC to human Raji cells. Method: Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 microl of anti-CD74/APC at a 1:50 dilution (10 microg/ml). Cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.65 log10 fluorescent units when compared to Isoptype Control Mouse IgG2a/APC (#ANC-281-060). Binding was blocked when cells were pre-incubated 10 min with 20 microl of 0.5 mg/ml anti-CD30 antibody (#ANC-179-020).
Binding of anti-CD74/APC to human Raji cells. Method: Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 microl of anti-CD74/APC at a 1:50 dilution (10 microg/ml). Cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.65 log10 fluorescent units when compared to Isoptype Control Mouse IgG2a/APC (#ANC-281-060). Binding was blocked when cells were pre-incubated 10 min with 20 microl of 0.5 mg/ml anti-CD30 antibody (#ANC-179-020).

anti-CD74 (human), mAb (M-B741) (APC)

ANC-226-060
Ancell Corporation
Product group Antibodies
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD74 (human), mAb (M-B741) (APC)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Scientific Description
    Human CD74 is expressed intracellularly and on the cell surface of MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans cells and dendritic cells. CD74 has several important functions relating to antigen presentation by MHC Class II molecules. - Monoclonal Antibody. Antibody clone M-B741 recognizes the human CD74 molecule. Isotype: Mouse IgG2a. Immunogen: RPMI 1788 cell line. Applications: FACS. Human CD74 is expressed intracellularly and on the cell surface of MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans cells and dendritic cells. CD74 has several important functions relating to antigen presentation by MHC Class II molecules.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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