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anti-CD74 (human), mAb (M-B741) (FITC)

Research Use Only
ANC-226-040
Ancell Corporation
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Ancell Corporation
  • Product Name
    anti-CD74 (human), mAb (M-B741) (FITC)
  • Delivery Days Customer
    10
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M-B741
  • Conjugate
    FITC
  • Formulation
    Liquid
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human CD74 is expressed intracellularly and on the cell surface of MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans cells and dendritic cells. CD74 has several important functions relating to antigen presentation by MHC Class II molecules. - Monoclonal Antibody. Isotype: Mouse IgG2a. Clone: M-B741. Applications: FACS. 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). Human CD74 is expressed intracellularly and on the cell surface of MHC Class II positive cells such as B cells, monocytes, macrophages, Langerhans cells and dendritic cells. CD74 has several important functions relating to antigen presentation by MHC Class II molecules.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Binding of anti-CD74/APC to human Raji cells. Method: Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 microl of anti-CD74/APC at a 1:50 dilution (10 microg/ml). Cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.65 log10 fluorescent units when compared to Isoptype Control Mouse IgG2a/APC (#ANC-281-060). Binding was blocked when cells were pre-incubated 10 min with 20 microl of 0.5 mg/ml anti-CD30 antibody (#ANC-179-020).
Product group Antibodies
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Product group Antibodies
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ApplicationsFlow Cytometry
ReactivityHuman
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