Anti-CD80 [RM80]

Absolute Antibody

Anti-CD80 [RM80]

9

This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) gammadelta T cell subsets (Ribot et al, 2012). Furthermore, this antibody has been used, together with the anti-CD86 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005).

Functional Assay, Flow Cytometry, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

RM80

Cd80

CD80 antigen

activation B7-1 antigen; B7 protein; B71; Cd28; Cd28l; Ly5; Ly-5; Ly53; Ly-53; MIC17; T-lymphocyte activation antigen CD80; TSA1

Mouse

IgG2b

T-lymphocyte activation antigen CD80

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203