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ProductsBack/GenieFluor Violet 450 Anti-Mouse F4/80 Antibody [CI:A3-1]

GenieFluor Violet 450 Anti-Mouse F4/80 Antibody [CI:A3-1]

AGEL2946
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd80
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Overview

  • Supplier
    Assay Genie
  • Product Name
    EV450 Anti-Mouse CD80 Antibody [16-10A1]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID12519
  • Target name
    Cd80
  • Target description
    CD80 antigen
  • Target synonyms
    activation B7-1 antigen; B7 protein; B71; Cd28; Cd28l; Ly5; Ly-5; Ly53; Ly-53; MIC17; T-lymphocyte activation antigen CD80; TSA1
  • Host
    Hamster
  • Protein IDQ00609
  • Protein Name
    T-lymphocyte activation antigen CD80
  • Shelf life instruction
    12 months
  • Source
    Armenian Hamster
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd80 using anti-Cd80 antibody (A00196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd80 antigen affinity purified polyclonal antibody (Catalog # A00196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd80 at approximately 70 kDa. The expected band size for Cd80 is at 33 kDa.
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