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ProductsBack/Anti-CD90 [F15-42-1-5], Mouse IgG1, kappa

Anti-CD90 [F15-42-1-5], Mouse IgG1, kappa

AB04289-1.1
Absolute Antibody
ApplicationsFlow Cytometry, RadioImmunoAssay
Product group Antibodies
TargetTHY1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD90 [F15-42-1-5], Mouse IgG1, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original version of the antibody was used for flow cytometry on fresh AMSCs to determine the AMSC phenotype (Dudakovic et al., 2013; PMID:24905804), and on hES cells and hMSC cells (Chen et al., 2003; PMID:12634409). The original antibody was used for a radioimmunoassay on human brain tissue, mouse brain tissue and mouse kidneys. Here it was able to show CD90 in these tissues without cross-reactivity to other proteins (Kollias et al., 1987; PMID:2888537). The original antibody was used for immunofluorescence on DPSC cells to phenotype them (Pilbauerova et al., 2019; PMID:31638559).
  • Applications
    Flow Cytometry, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F15-42-1-5
  • Gene ID7070
  • Target name
    THY1
  • Target description
    Thy-1 cell surface antigen
  • Target synonyms
    CD90; CDw90; thy-1 antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04216
  • Protein Name
    Thy-1 membrane glycoprotein
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 24 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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