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Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in neuropil.

Anti-THY1 Antibody

AMAB90844
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTHY1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-THY1
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL1028
  • Conjugate
    Unconjugated
  • Gene ID7070
  • Target name
    THY1
  • Target description
    Thy-1 cell surface antigen
  • Target synonyms
    CD90; CDw90; thy-1 antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP04216
  • Protein Name
    Thy-1 membrane glycoprotein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 24 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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