Anti-CD99 [12E7]

Absolute Antibody

Anti-CD99 [12E7]

7

The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewings sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). This antibody was also used in a study relating to the cell surface expression MIC2 antigen on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting (PMID: 7506950).

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, RadioImmunoAssay

IF; RIA; WB; ICC; IHC; FC

Research Use Only

Monoclonal

12E7

CD99

CD99 molecule (Xg blood group)

antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; CD99 antigen; cell surface antigen 12E7; cell surface antigen HBA-71; cell surface antigen O13; E2 antigen; HBA71; MIC2; MIC2 (monoclonal antibody 12E7); MIC2X; MIC2Y; MSK5X; surface antigen MIC2; T-cell surface glycoprotein E2

Mouse

IgG1

CD99 antigen

Human

The original antibody was generated by immunizing BALB/c mice intraperitoneally with glutaraldehyde-fixed human acute lymphocytic leukemia (ALL) cells obtained from peripheral blood of a child.

-20°C,2°C to 8°C

12352203