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Immunohistochemical staining of human ovarian cancer (mucinous carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemical staining of human ovarian cancer (mucinous carcinoma) shows moderate membranous positivity in tumor cells.
Immunohistochemical staining of human ovarian cancer (mucinous carcinoma) shows moderate membranous positivity in tumor cells.

Anti-CD99 Antibody

AMAB91823
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD99
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD99
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL12929
  • Conjugate
    Unconjugated
  • Gene ID4267
  • Target name
    CD99
  • Target description
    CD99 molecule (Xg blood group)
  • Target synonyms
    HBA71, MIC2, MIC2X, MIC2Y, MSK5X, CD99 antigen, E2 antigen, MIC2 (monoclonal antibody 12E7), T-cell surface glycoprotein E2, antigen identified by monoclonal 12E7, Y homolog, antigen identified by monoclonal antibodies 12E7, F21 and O13, cell surface antigen 12E7, cell surface antigen HBA-71, cell surface antigen O13, surface antigen MIC2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP14209
  • Protein Name
    CD99 antigen
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of CD99 using anti-CD99 antibody (A01724-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD99 antigen affinity purified polyclonal antibody (Catalog # A01724-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD99 at approximately 28 kDa. The expected band size for CD99 is at 28 kDa.
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