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Anti-CDC42 antibody, PA1366, Western blotting Lane 1: Recombinant Human CDC42 Protein 10ng Lane 2: Recombinant Human CDC42 Protein 5ng Lane 3: Recombinant Human CDC42 Protein 2.5ng
Anti-CDC42 antibody, PA1366, Western blotting Lane 1: Recombinant Human CDC42 Protein 10ng Lane 2: Recombinant Human CDC42 Protein 5ng Lane 3: Recombinant Human CDC42 Protein 2.5ng
Anti-CDC42 antibody, PA1366, Western blotting Lane 1: Recombinant Human CDC42 Protein 10ng Lane 2: Recombinant Human CDC42 Protein 5ng Lane 3: Recombinant Human CDC42 Protein 2.5ng

Anti-CDC42 Antibody

PA1366
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Chicken, Human, Mouse, Rat
TargetCDC42
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CDC42 Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHP, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID998
  • Target name
    CDC42
  • Target description
    cell division cycle 42
  • Target synonyms
    CDC42Hs, G25K, TKS, cell division control protein 42 homolog, G25K GTP-binding protein, GTP binding protein, 25kDa, dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)), dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)), growth-regulating protein, small GTP binding protein CDC42
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60953
  • Protein Name
    Cell division control protein 42 homolog
  • Scientific Description
    Boster Bio Anti-CDC42 Antibody catalog # PA1366. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Bovine, Chicken, Human, Mouse, Rat
  • Reactivity Supplier
    Bovine, Human, Mouse, Rat, Chicken
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shi C, Luo W, Sun C, et al. The miR-29 family members induce glioblastoma cell apoptosis by targeting cell division cycle 42 in a p53-dependent manner. Eur J Clin Invest. 2023,53(6):e13964. doi: 10.1111/eci.13964
    Read this paper
  • Wen D, Cui J, Li P, et al. Syndecan-4 assists Mycobacterium tuberculosis entry into lung epithelial cells by regulating the Cdc42, N-WASP, and Arp2/3 signaling pathways. Microbes Infect. 2022,24(4):104931. doi: 10.1016/j.micinf.2022.104931
    Read this paper
  • Guo Y, Wang J, Zhou K, et al. Cytotoxic necrotizing factor 1 promotes bladder cancer angiogenesis through activating RhoC. FASEB J. 2020,34(6):7927-7940. doi: 10.1096/fj.201903266RR
    Read this paper
  • Zhuang TT, Pan C, Chen JJ, et al. Chronic asthma-induced behavioral and hippocampal neuronal morphological changes are concurrent with BDNF, cofilin1 and Cdc42/RhoA alterations in immature mice. Brain Res Bull. 2018,143:194-206. doi: 10.1016/j.brainresbull.2018.09.006
    Read this paper
  • Han F, Zhuang TT, Chen JJ, et al. Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and RAC1/CDC42 alterations in rats. PLoS One. 2017,12(9):e0185102. doi: 10.1371/journal.pone.0185102
    Read this paper

Datasheet

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MSDS

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Figure 1. Western blot analysis of CDC42 using anti-CDC42 antibody (A00119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 antigen affinity purified polyclonal antibody (Catalog # A00119-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42 at approximately 21 kDa. The expected band size for CDC42 is at 21 kDa.
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