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Anti-CDC6 Antibody

144-65338
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetCDC6
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CDC6 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Gene ID990
  • Target name
    CDC6
  • Target description
    cell division cycle 6
  • Target synonyms
    CDC18L, HsCDC18, HsCDC6, MGORS5, cell division control protein 6 homolog, CDC6 cell division cycle 6 homolog, CDC6-related protein, cdc18-related protein, cell division cycle 6 homolog, p62(cdc6)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99741
  • Protein Name
    Cell division control protein 6 homolog
  • Scientific Description
    CDC6 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cdc6 using anti-Cdc6 antibody (PB9531). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: JURKAT Whole Cell Lysate at 40ug, Lane 3: MM231 Whole Cell Lysate at 40ug, Lane 4: HT1080 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc6 antigen affinity purified polyclonal antibody (Catalog # PB9531) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc6 at approximately 63 kDa. The expected band size for Cdc6 is at 63 kDa.
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