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ProductsBack/Phospho-CDC6 (Ser106) Antibody (YA1016, Rabbit)

Phospho-CDC6 (Ser106) Antibody (YA1016, Rabbit)

HY-P81295
MedChem Express
TargetCDC6
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Phospho-CDC6 (Ser106) Antibody (YA1016, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID990
  • Target name
    CDC6
  • Target description
    cell division cycle 6
  • Target synonyms
    CDC18L; cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; cell division control protein 6 homolog; cell division cycle 6 homolog; HsCDC18; HsCDC6; MGORS5; p62(cdc6)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Phospho-CDC6 (Ser106) Antibody (YA1016) is a non-conjugated IgG antibody, targeting Phospho-CDC6 (Ser106), with a predicted molecular weight of 63 kDa (observed band size: 63 kDa). Phospho-CDC6 (Ser106) Antibody (YA1016) can be used for WB experiment in human, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cdc6 using anti-Cdc6 antibody (PB9531). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: JURKAT Whole Cell Lysate at 40ug, Lane 3: MM231 Whole Cell Lysate at 40ug, Lane 4: HT1080 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc6 antigen affinity purified polyclonal antibody (Catalog # PB9531) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc6 at approximately 63 kDa. The expected band size for Cdc6 is at 63 kDa.
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