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Immunohistochemical staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemical staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemical staining of human endometrium shows no positivity in glandular cells as expected.

Anti-CDH15 Antibody

HPA009139
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCDH15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CDH15
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1013
  • Target name
    CDH15
  • Target description
    cadherin 15
  • Target synonyms
    cadherin 15, type 1, M-cadherin (myotubule); cadherin-14; cadherin-15; cadherin-3; CDH14; CDH3; CDHM; MCAD; MRD3; muscle-cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55291
  • Protein Name
    Cadherin-15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of M Cadherin/CDH15 using anti-M Cadherin/CDH15 antibody (A10888-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-M Cadherin/CDH15 antigen affinity purified polyclonal antibody (Catalog # A10888-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for M Cadherin/CDH15 at approximately 130 kDa. The expected band size for M Cadherin/CDH15 is at 89 kDa.
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