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Western Blot analysis of HepG2 cells using M-cadherin Polyclonal Antibody
Western Blot analysis of HepG2 cells using M-cadherin Polyclonal Antibody
Western Blot analysis of HepG2 cells using M-cadherin Polyclonal Antibody

CDH15 Antibody

CSB-PA003206
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCDH15
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Overview

  • Supplier
    Cusabio
  • Product Name
    CDH15 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1013
  • Target name
    CDH15
  • Target description
    cadherin 15
  • Target synonyms
    CDH14, CDH3, CDHM, MCAD, MRD3, cadherin-15, cadherin 15, type 1, M-cadherin (myotubule), cadherin-14, cadherin-3, muscle-cadherin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55291
  • Protein Name
    Cadherin-15
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of M Cadherin/CDH15 using anti-M Cadherin/CDH15 antibody (A10888-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-M Cadherin/CDH15 antigen affinity purified polyclonal antibody (Catalog # A10888-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for M Cadherin/CDH15 at approximately 130 kDa. The expected band size for M Cadherin/CDH15 is at 89 kDa.
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