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Anti-CDT2 Antibody

Anti-CDT2 Antibody

A365
Antibodies.com
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-CDT2 Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to CDT2.
  • Reactivity
    Hamster, Human, Mouse, Rat
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of DTL using anti-DTL antibody (M01255). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTL antigen affinity purified monoclonal antibody (M01255) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DTL at approximately 95 kDa. The expected band size for DTL is at 79 kDa.
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