Anti-CEA [CH1A1A (2F1, CH1A1A-2F1)]

AB02721-1.1-BT
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCEACAM5
Overview
- SupplierAbsolute Antibody
- Product NameAnti-CEA [CH1A1A (2F1, CH1A1A-2F1)]
- Delivery Days Customer9
- Antibody SpecificityThis antibody is capable of recognizing a membrane-proximal epitope in the B3 domain of CEA (CEACAM5, Cd66e), whereas it does not recognize shed CEA or soluble CEA as found in the circulation (PMID: 19904275; 7514303; 11517330).
- Application Supplier NoteCH1A1A is a humanized, affinity matured, and stability-engineered version derived from the murine parental PR1A3 antibody, first disclosed in PMID: 2434440. This antibody is also basis of CEA TCB (RG7802), a CEA-CD3 T cell bispecific antibody currently in Phase 1/1b clinical trials (PMID: 27622073; 27117182; 26861458). This antibody was also used in the generation of cergutuzumab amunaleukin (CEA-IL2v, RG7813), a monomeric CEA-targeted immunocytokine, that comprises a single IL-2 variant (IL2v) moiety with abolished CD25 binding, fused to the C-terminus of a high affinity, bivalent carcinoembryonic antigen (CEA)-specific antibody devoid of Fc mediated effector functions. The binding affinity of this antibody for CEA was tested using ELISA. This antibody can also be used in the immunohistochemical analysis of CEA expression by various tumors (PMID: 28405498). This antibody was also used to study the surface expression heterogeneity of CEA in cells extracted from patient derived colorectal cancer organoids (PDOs) using flow cytometry (PMID: 30982469).
- ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDCH1A1A (2F1, CH1A1A-2F1)
- Gene ID1048
- Target nameCEACAM5
- Target descriptionCEA cell adhesion molecule 5
- Target synonymscarcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
- HostMouse
- IsotypeIgG1
- Protein IDP06731
- Protein NameCarcinoembryonic antigen-related cell adhesion molecule 5
- Scientific DescriptionThis chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203