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ProductsBack/Anti-CEA [CH1A1A (2F1, CH1A1A-2F1)]

Anti-CEA [CH1A1A (2F1, CH1A1A-2F1)]

Ab02721-23.0
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCEACAM5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CEA [CH1A1A (2F1, CH1A1A-2F1)]
  • Delivery Days Customer
    7
  • Antibody Specificity
    This antibody is capable of recognizing a membrane-proximal epitope in the B3 domain of CEA (CEACAM5, Cd66e), whereas it does not recognize shed CEA or soluble CEA as found in the circulation (PMID: 19904275; 7514303; 11517330).
  • Application Supplier Note
    CH1A1A is a humanized, affinity matured, and stability-engineered version derived from the murine parental PR1A3 antibody, first disclosed in PMID: 2434440. This antibody is also basis of CEA TCB (RG7802), a CEA-CD3 T cell bispecific antibody currently in Phase 1/1b clinical trials (PMID: 27622073; 27117182; 26861458). This antibody was also used in the generation of cergutuzumab amunaleukin (CEA-IL2v, RG7813), a monomeric CEA-targeted immunocytokine, that comprises a single IL-2 variant (IL2v) moiety with abolished CD25 binding, fused to the C-terminus of a high affinity, bivalent carcinoembryonic antigen (CEA)-specific antibody devoid of Fc mediated effector functions. The binding affinity of this antibody for CEA was tested using ELISA. This antibody can also be used in the immunohistochemical analysis of CEA expression by various tumors (PMID: 28405498). This antibody was also used to study the surface expression heterogeneity of CEA in cells extracted from patient derived colorectal cancer organoids (PDOs) using flow cytometry (PMID: 30982469).
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; FC; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CH1A1A (2F1, CH1A1A-2F1)
  • Gene ID1048
  • Target name
    CEACAM5
  • Target description
    CEA cell adhesion molecule 5
  • Target synonyms
    carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06731
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 5
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original murine antibody PR.1A3 was generated by immunizing BALB/c mice with mucosal scrapings and cell membrane preparations from normal large intestine. Booster dose was given with same antigen or in one case boosting was also performed with HT29 colon carcinoma cells. CH1A1A was later one generated for affinity maturation and stability enhancement by phage display.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203