Anti-CENP-B [5D1]

Absolute Antibody

Anti-CENP-B [5D1]

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This antibody binds human major centromere autoantigen B (CENP-B). It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization doma

The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human CENP-B in a western blot assay (PMID: 18062792).

Western Blot, ELISA

ELISA; WB

Research Use Only

Monoclonal

5D1

CENPB

centromere protein B

CENP-B; centromere autoantigen B; centromere protein B, 80kDa; major centromere autoantigen B

Human

IgG1

Major centromere autoantigen B

This full-length chimeric human antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.

-20°C,2°C to 8°C

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