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ProductsBack/Anti-CENP-B [5D1]

Anti-CENP-B [5D1]

AB03244-30.11-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCENPB
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CENP-B [5D1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human CENP-B in a western blot assay (PMID: 18062792).
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D1
  • Gene ID1059
  • Target name
    CENPB
  • Target description
    centromere protein B
  • Target synonyms
    major centromere autoantigen B, CENP-B, centromere autoantigen B, centromere protein B, 80kDa
  • Protein IDP07199
  • Protein Name
    Major centromere autoantigen B
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CENPB using anti-CENPB antibody (A05140-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPB antigen affinity purified polyclonal antibody (Catalog # A05140-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPB at approximately 80 kDa. The expected band size for CENPB is at 80 kDa.
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