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Immunohistochemical staining of human fallopian tube shows strong positivity in cilia in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong positivity in cilia in glandular cells.

Anti-CEP164 Antibody

HPA037606
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCEP164
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CEP164
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22897
  • Target name
    CEP164
  • Target description
    centrosomal protein 164
  • Target synonyms
    centrosomal protein 164kDa; centrosomal protein of 164 kDa; NPHP15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPV0
  • Protein Name
    Centrosomal protein of 164 kDa
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA892491LA01HU diluted at 1:600 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of CEP164 using anti-CEP164 antibody (A05971-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP164 antigen affinity purified polyclonal antibody (Catalog # A05971-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP164 at approximately 164 kDa. The expected band size for CEP164 is at 164 kDa.
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