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ProductsBack/CEP164 antibody [13]

CEP164 antibody [13]

GTX70094
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCEP164
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Overview

  • Supplier
    GeneTex
  • Product Name
    CEP164 antibody [13]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    13
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22897
  • Target name
    CEP164
  • Target description
    centrosomal protein 164
  • Target synonyms
    centrosomal protein 164kDa; centrosomal protein of 164 kDa; NPHP15
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UPV0
  • Protein Name
    Centrosomal protein of 164 kDa
  • Scientific Description
    This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cep164 is a mediator protein required for the maintenance of genomic stability through modulation of MDC1, RPA, and CHK1. Sivasubramaniam S et al., 2008 Mar 1, Genes Dev
    Read more

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Figure 1. Western blot analysis of CEP164 using anti-CEP164 antibody (A05971-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP164 antigen affinity purified polyclonal antibody (Catalog # A05971-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP164 at approximately 164 kDa. The expected band size for CEP164 is at 164 kDa.
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