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Anti-CETP Antibody

144-01355
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCETP
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CETP Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1071
  • Target name
    CETP
  • Target description
    cholesteryl ester transfer protein
  • Target synonyms
    BPIFF, HDLCQ10, cholesteryl ester transfer protein, BPI fold containing family F, cholesteryl ester transfer protein plasma, lipid transfer protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11597
  • Protein Name
    Cholesteryl ester transfer protein
  • Scientific Description
    CETP Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-RA005267A0HU diluted at 1:115 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of CETP using anti-CETP antibody (A00309-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CETP antigen affinity purified polyclonal antibody (Catalog # A00309-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CETP at approximately 75 kDa. The expected band size for CETP is at 55 kDa.
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