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Anti-cFos [S1-71]

Ab02293-10.0
Absolute Antibody
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFOS
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-cFos [S1-71]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for detection and analysis of cFos by ELISA, immunofluorescence, immunohistochemistry and western blot.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; IF; IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    S1-71
  • Gene ID2353
  • Target name
    FOS
  • Target description
    Fos proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, C-FOS, p55, protein c-Fos, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, Fos proto-oncogene, AP-1 trancription factor subunit, G0/G1 switch regulatory protein 7, activator protein 1, cellular oncogene c-fos, proto-oncogene c-Fos, transcription factor AP-1 subunit c-Fos
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP01100
  • Protein Name
    Proto-oncogene c-Fos
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    human
  • Reactivity Supplier Note
    This antibody was generated against the proto-oncogene c-Fos.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
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