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Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-0469R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-0469R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spinal cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-fos Polyclonal Antibody, Unconjugated (bs-0469R) at 1:400 overnight at 4°C, DAB staining.

c-fos Polyclonal Antibody

bs-0469R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFOS
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Overview

  • Supplier
    Bioss
  • Product Name
    c-fos Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2353
  • Target name
    FOS
  • Target description
    Fos proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, C-FOS, p55, protein c-Fos, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, Fos proto-oncogene, AP-1 trancription factor subunit, G0/G1 switch regulatory protein 7, activator protein 1, cellular oncogene c-fos, proto-oncogene c-Fos, transcription factor AP-1 subunit c-Fos
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01100
  • Protein Name
    Protein c-Fos
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang Q, Kuang G, He S, et al. Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer. Nano Lett. 2020,20(5):3039-3049. doi: 10.1021/acs.nanolett.9b04981
    Read this paper
  • Ma Q, Liang M, Tang X, et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019,11(8):5008-5018.
    Read this paper
  • Xiao J, Song M, Li F, et al. Effects of GABA microinjection into dorsal raphe nucleus on behavior and activity of lateral habenular neurons in mice. Exp Neurol. 2017,298(Pt A):23-30. doi: 10.1016/j.expneurol.2017.08.012
    Read this paper
  • Wu S, Cao J, Zhang T, et al. Electroacupuncture Ameliorates the Coronary Occlusion Related Tachycardia and Hypotension in Acute Rat Myocardial Ischemia Model: Potential Role of Hippocampus. Evid Based Complement Alternat Med. 2015,2015:925987. doi: 10.1155/2015/925987
    Read this paper

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Figure 1. Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
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