More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-CHEK1 Antibody

Anti-CHEK1 Antibody

144-07653
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHEK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CHEK1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1111
  • Target name
    CHEK1
  • Target description
    checkpoint kinase 1
  • Target synonyms
    CHK1, OZEMA21, serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Checkpoint, S. pombe, homolog of, 1, Chk1-S, cell cycle checkpoint kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14757
  • Protein Name
    Serine/threonine-protein kinase Chk1
  • Scientific Description
    CHEK1 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of K562 cells using Chk1 Polyclonal Antibody
Product group Antibodies
Cusabio
CHEK1 AntibodyCSB-PA001623
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCHEK1
  • SizePrice
Product group Antibodies
Biomatik
Chek1 Recombinant AntibodyCAC12088
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCHEK1
  • SizePrice
Anti-Chk1 Antibody
Product group Antibodies
Antibodies.com
Anti-Chk1 AntibodyA100008
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
  • SizePrice
Figure 1. Western blot analysis of Chk1/CHEK1 using anti-Chk1/CHEK1 antibody (A01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1/CHEK1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1/CHEK1 at approximately 54 kDa. The expected band size for Chk1/CHEK1 is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-Chk1/CHEK1 Antibody Picoband(r)A01060-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetCHEK1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CHEK1 / CHK1 AntibodyLS-C761161
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetCHEK1
  • SizePrice
Product group Antibodies
MedChem Express
Chk1 Antibody (YA505, Rabbit)HY-P80082
TargetCHEK1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-CHEK1EB08452
ApplicationsWestern Blot, ELISA
ReactivityBovine, Human, Mouse, Rat
TargetCHEK1
  • SizePrice
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-CHEK1 AntibodyHPA044364
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetCHEK1
  • SizePrice
Product group Antibodies
References
Bioss
phospho-CHEK1 (Ser317) Polyclonal AntibodyBS-5251R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityEquine, Human, Mouse, Porcine, Rabbit, Rat
TargetCHEK1
  • SizePrice
Untreated (–) and treated (+) C2C12 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser345) antibody [C1C2], Internal (GTX100065) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
Chk1 (phospho Ser345) antibody [C1C2], InternalGTX100065
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetCHEK1
  • SizePrice