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ProductsBack/phospho-CHEK1 (Ser317) Polyclonal Antibody

phospho-CHEK1 (Ser317) Polyclonal Antibody

BS-5251R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCHEK1
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Overview

  • Supplier
    Bioss
  • Product Name
    phospho-CHEK1 (Ser317) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1111
  • Target name
    CHEK1
  • Target description
    checkpoint kinase 1
  • Target synonyms
    CHK1, OZEMA21, serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Checkpoint, S. pombe, homolog of, 1, Chk1-S, cell cycle checkpoint kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14757
  • Protein Name
    Serine/threonine-protein kinase Chk1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Guo H, Cui H, Peng X, et al. Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney. Oncotarget. 2015,6(34):35964-77. doi: 10.18632/oncotarget.5934
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Chk1/CHEK1 using anti-Chk1/CHEK1 antibody (A01060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk1/CHEK1 antigen affinity purified polyclonal antibody (Catalog # A01060) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk1/CHEK1 at approximately 54 kDa. The expected band size for Chk1/CHEK1 is at 54 kDa.
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