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Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.

Anti-CHTOP Antibody

HPA030540
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCHTOP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CHTOP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26097
  • Target name
    CHTOP
  • Target description
    chromatin target of PRMT1
  • Target synonyms
    C10orf77; C1orf77; chromatin target of PRMT1 protein; FL-SRAG; FOP; friend of PRMT1 protein; pp7704; small protein rich in arginine and glycine; SRAG; SRAG-3; SRAG-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3Y2
  • Protein Name
    Chromatin target of PRMT1 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cells in granular layer.
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Figure 1. Western blot analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse spleen tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf77/FOP/CHTOP antigen affinity purified polyclonal antibody (Catalog # A07770-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1orf77/FOP/CHTOP at approximately 28 kDa. The expected band size for C1orf77/FOP/CHTOP is at 26 kDa.
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