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Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-CIB1 Antibody

HPA042413
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCIB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CIB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10519
  • Target name
    CIB1
  • Target description
    calcium and integrin binding 1
  • Target synonyms
    calcium and integrin binding 1 (calmyrin); calcium and integrin-binding protein 1; CIB; CIBP; DNA-dependent protein kinase interacting protein; DNA-PK interaction protein; DNA-PKcs-interacting protein; KIP1; PRKDCIP; SIP2-28; SNK-interacting protein 2-28; testicular secretory protein Li 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99828
  • Protein Name
    Calcium and integrin-binding protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of CIB1 using anti-CIB1 antibody (PB9489). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brian tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIB1 antigen affinity purified polyclonal antibody (Catalog # PB9489) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIB1 at approximately 22 kDa. The expected band size for CIB1 is at 27 kDa.
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