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Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.

Anti-CLASP2 Antibody

HPA067071
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCLASP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CLASP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23122
  • Target name
    CLASP2
  • Target description
    cytoplasmic linker associated protein 2
  • Target synonyms
    CLIP-associating protein 2; CLIP-associating protein CLASP2; multiple asters (Mast)-like homolog 2; protein Orbit homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75122
  • Protein Name
    CLIP-associating protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CLASP2 using anti-CLASP2 antibody (A03579-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLASP2 antigen affinity purified polyclonal antibody (Catalog # A03579-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLASP2 at approximately 165 kDa. The expected band size for CLASP2 is at 141 kDa.
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