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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA005472LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA005472LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA005472LA01HU at dilution of 1:100

CLASP2 Antibody

CSB-PA005472LA01HU
Cusabio
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCLASP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLASP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23122
  • Target name
    CLASP2
  • Target description
    cytoplasmic linker associated protein 2
  • Target synonyms
    CLIP-associating protein 2, CLIP-associating protein CLASP2, multiple asters (Mast)-like homolog 2, protein Orbit homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75122
  • Protein Name
    CLIP-associating protein 2
  • Scientific Description
    Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CLASP2 using anti-CLASP2 antibody (A03579-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLASP2 antigen affinity purified polyclonal antibody (Catalog # A03579-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLASP2 at approximately 165 kDa. The expected band size for CLASP2 is at 141 kDa.
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