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ProductsBack/FluoSite(tm) Anti-Claudin-18 Isoform 2 Antibody, FITC-Labeled
Cell surface expression of CLDN18.2 in CLDN18.2 CHO Cell Line.
Cell surface expression of CLDN18.2 in CLDN18.2 CHO Cell Line.
Cell surface expression of CLDN18.2 in CLDN18.2 CHO Cell Line.

FluoSite(tm) Anti-Claudin-18 Isoform 2 Antibody, FITC-Labeled

101866
BPS Bioscience
ApplicationsFlow Cytometry
Product group Antibodies
TargetCLDN18
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    Anti-Claudin-18 Isoform 2 Antibody, FITC-Labeled
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    FITC
  • Estimated Purity
    ≥90%
  • Gene ID51208
  • Target name
    CLDN18
  • Target description
    claudin 18
  • Target synonyms
    claudin-18; SFTA5; SFTPJ; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP56856
  • Protein Name
    Claudin-18
  • Scientific Description
    This anti-Claudin-18 isoform 2 (CLDN18.2) antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for specific binding to human CLDN18.2 on the surface of CLDN18.2 CHO Recombinant Cell Line (BPS Bioscience #78533).
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin18/CLDN18 antigen affinity purified polyclonal antibody (Catalog # A09129-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin18/CLDN18 at approximately 30 kDa. The expected band size for Claudin18/CLDN18 is at 30 kDa.
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