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Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.

Anti-CLDN3 Antibody

HPA014361
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetCLDN3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CLDN3
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1365
  • Target name
    CLDN3
  • Target description
    claudin 3
  • Target synonyms
    C7orf1; claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-R2; CPE-receptor 2; CPETR2; HRVP1; RVP1; ventral prostate.1 protein homolog; ventral prostate.1-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15551
  • Protein Name
    Claudin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (A04393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 3/CLDN3 antigen affinity purified polyclonal antibody (Catalog # A04393-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 23KD. The expected band size for Claudin 3/CLDN3 is at 23KD.
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