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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Anti-CMBL Antibody

HPA036571
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCMBL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CMBL
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID134147
  • Target name
    CMBL
  • Target description
    carboxymethylenebutenolidase homolog
  • Target synonyms
    JS-1, carboxymethylenebutenolidase homolog, carboxymethylenebutenolidase homolog (Pseudomonas), carboxymethylenebutenolidase-like (Pseudomonas)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DG6
  • Protein Name
    Carboxymethylenebutenolidase homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (A04921-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # A04921-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD.
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