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Western blot All lanes: CMBL antibody at 1microg/ml Lane 1: Mouse kidney tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29, 25 kDa
Western blot All lanes: CMBL antibody at 1microg/ml Lane 1: Mouse kidney tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29, 25 kDa
Western blot All lanes: CMBL antibody at 1microg/ml Lane 1: Mouse kidney tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29, 25 kDa

CMBL Antibody

CSB-PA846604LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCMBL
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Overview

  • Supplier
    Cusabio
  • Product Name
    CMBL Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID134147
  • Target name
    CMBL
  • Target description
    carboxymethylenebutenolidase homolog
  • Target synonyms
    JS-1, carboxymethylenebutenolidase homolog, carboxymethylenebutenolidase homolog (Pseudomonas), carboxymethylenebutenolidase-like (Pseudomonas)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DG6
  • Protein Name
    Carboxymethylenebutenolidase homolog
  • Scientific Description
    Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (A04921-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: rat liver tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC134147/CMBL antigen affinity purified polyclonal antibody (Catalog # A04921-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOC134147/CMBL at approximately 28KD. The expected band size for LOC134147/CMBL is at 28KD.
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