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Immunohistochemical staining of human colon shows moderate positivity in extracellular matrix.
Immunohistochemical staining of human colon shows moderate positivity in extracellular matrix.
Immunohistochemical staining of human colon shows moderate positivity in extracellular matrix.

Anti-COL1A1 Antibody

HPA011795
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOL1A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-COL1A1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1277
  • Target name
    COL1A1
  • Target description
    collagen type I alpha 1 chain
  • Target synonyms
    CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, collagen alpha-1(I) chain, alpha-1 type I collagen, alpha1(I) procollagen, collagen Col1-ColIII-1, collagen Col1-ColIII-2, collagen alpha 1 chain type I, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, pro-alpha-1 collagen type 1, type I proalpha 1, type I procollagen alpha 1 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02452
  • Protein Name
    Collagen alpha-1(I) chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Collagen I using anti-Collagen I antibody (PB9938). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I antigen affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I at approximately 130-180 kDa. The expected band size for Collagen I is at 139 kDa.
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