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This image was kindly submitted by Kandy Velazquez of USC School of Medicine._x000D_ Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
This image was kindly submitted by Kandy Velazquez of USC School of Medicine._x000D_ Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.
This image was kindly submitted by Kandy Velazquez of USC School of Medicine._x000D_ Mouse adipose tissue lysates probed with Collagen I Polyclonal Antibody, unconjugated (bs-10423R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at room temperature.

Collagen I Polyclonal Antibody

BS-10423R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL1A1
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Overview

  • Supplier
    Bioss
  • Product Name
    Collagen I Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:200-1000), IHC-P(1:200-1000), IHC-F(1:200-1000), IF(1:200-1000), ICC/IF(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1277
  • Target name
    COL1A1
  • Target description
    collagen type I alpha 1 chain
  • Target synonyms
    CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, collagen alpha-1(I) chain, alpha-1 type I collagen, alpha1(I) procollagen, collagen Col1-ColIII-1, collagen Col1-ColIII-2, collagen alpha 1 chain type I, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, pro-alpha-1 collagen type 1, type I proalpha 1, type I procollagen alpha 1 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02452
  • Protein Name
    Collagen alpha-1(I) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Gao X, Zhong S, Tong Y, et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020,216(3):152831. doi: 10.1016/j.prp.2020.152831
    Read this paper
  • Yang J, Tao Q, Zhou Y, et al. MicroRNA-708 represses hepatic stellate cells activation and proliferation by targeting ZEB1 through Wnt/β-catenin pathway. Eur J Pharmacol. 2020,871:172927. doi: 10.1016/j.ejphar.2020.172927
    Read this paper
  • Han B, Lv Z, Zhang X, et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2020,259:113870. doi: 10.1016/j.envpol.2019.113870
    Read this paper
  • Lv Y, Bing Q, Lv Z, et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2020,705:135915. doi: 10.1016/j.scitotenv.2019.135915
    Read this paper
  • Wang W, Jia YJ, Yang YL, et al. LncRNA GAS5 exacerbates renal tubular epithelial fibrosis by acting as a competing endogenous RNA of miR-96-5p. Biomed Pharmacother. 2020,121:109411. doi: 10.1016/j.biopha.2019.109411
    Read this paper
  • Liu C, Li Y, Yang Z, et al. Kartogenin enhances the therapeutic effect of bone marrow mesenchymal stem cells derived exosomes in cartilage repair. Nanomedicine (Lond). 2020,15(3):273-288. doi: 10.2217/nnm-2019-0208
    Read this paper
  • Yu B, Yu M, Zhang H, et al. Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Exp Mol Pathol. 2020,112:104346. doi: 10.1016/j.yexmp.2019.104346
    Read this paper
  • Hu N, Wang C, Dai X, et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2020,248:112361. doi: 10.1016/j.jep.2019.112361
    Read this paper
  • Wu C, Chen W, Ding H, et al. Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro. Life Sci. 2019,239:116881. doi: 10.1016/j.lfs.2019.116881
    Read this paper
  • Chen X, Li XF, Chen Y, et al. Hesperetin derivative attenuates CCl(4)-induced hepatic fibrosis and inflammation by Gli-1-dependent mechanisms. Int Immunopharmacol. 2019,76:105838. doi: 10.1016/j.intimp.2019.105838
    Read this paper

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Figure 1. Western blot analysis of Collagen I using anti-Collagen I antibody (PB9938). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skin tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse skin tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I antigen affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I at approximately 130-180 kDa. The expected band size for Collagen I is at 139 kDa.
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