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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in endothelial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in endothelial cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in endothelial cells.

Anti-COL4A2 Antibody

HPA069337
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOL4A2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-COL4A2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1284
  • Target name
    COL4A2
  • Target description
    collagen type IV alpha 2 chain
  • Target synonyms
    BSVD2, ICH, POREN2, collagen alpha-2(IV) chain, canstatin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08572
  • Protein Name
    Collagen alpha-2(IV) chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A2 antibody [N2N3] (GTX102422) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Figure 1. IHC analysis of COL4A2 using anti-COL4A2 antibody (A02453-1). COL4A2 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-COL4A2 Antibody (A02453-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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