Rabbit anti Collagen IV

Nordic-MUbio

Rabbit anti Collagen IV

7

Polyclonal type IV collagen recognizes the extracellular - basement membranes.

Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration; 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting

Primary antibodies

Research Use Only

Polyclonal

COL4A2

collagen type IV alpha 2 chain

BSVD2; canstatin; collagen alpha-2(IV) chain; ICH; POREN2

Rabbit

Collagen alpha-2(IV) chain

This polyclonal type IV collagen antibody is a rabbit polyclonal antibody. The rabbits were immunized with isolated human type IV collagen. The immunogen has been isolated from human placenta.

Bovine, Human, Porcine, Rat

Bovine;Human;Rat;Swine

12352203