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Anti-COLGALT1 Antibody

144-64504
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOLGALT1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-COLGALT1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID79709
  • Target name
    COLGALT1
  • Target description
    collagen beta(1-O)galactosyltransferase 1
  • Target synonyms
    BSVD3, ColGalT 1, GLT25D1, procollagen galactosyltransferase 1, glycosyltransferase 25 domain containing 1, glycosyltransferase 25 family member 1, hydroxylysine galactosyltransferase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBJ5
  • Protein Name
    Procollagen galactosyltransferase 1
  • Scientific Description
    COLGALT1 pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLT25D1/COLGALT1 using anti-GLT25D1/COLGALT1 antibody (A12583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLT25D1/COLGALT1 antigen affinity purified polyclonal antibody (Catalog # A12583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLT25D1/COLGALT1 at approximately 85 kDa. The expected band size for GLT25D1/COLGALT1 is at 72 kDa.
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ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCOLGALT1
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ReactivityHuman
TargetCOLGALT1
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Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Human THP-1 cell lysates probed with COLGALT1 /GLT25D1Polyclonal Antibody, Unconjugated (bs-13378R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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