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Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.

Anti-COLGALT1 Antibody

HPA047821
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOLGALT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-COLGALT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79709
  • Target name
    COLGALT1
  • Target description
    collagen beta(1-O)galactosyltransferase 1
  • Target synonyms
    BSVD3, ColGalT 1, GLT25D1, procollagen galactosyltransferase 1, glycosyltransferase 25 domain containing 1, glycosyltransferase 25 family member 1, hydroxylysine galactosyltransferase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBJ5
  • Protein Name
    Procollagen galactosyltransferase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GLT25D1/COLGALT1 using anti-GLT25D1/COLGALT1 antibody (A12583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLT25D1/COLGALT1 antigen affinity purified polyclonal antibody (Catalog # A12583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLT25D1/COLGALT1 at approximately 85 kDa. The expected band size for GLT25D1/COLGALT1 is at 72 kDa.
Product group Antibodies
Boster Bio
Anti-GLT25D1/COLGALT1 Antibody Picoband(r)A12583-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCOLGALT1
  • SizePrice
Atlas Antibodies
PrEST Antigen COLGALT1APREST82677
Protein IDQ8NBJ5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-COLGALT1 Antibody144-64504
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCOLGALT1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
COLGALT1 / GLT25D1 AntibodyLS-C665871
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetCOLGALT1
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Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Human THP-1 cell lysates probed with COLGALT1 /GLT25D1Polyclonal Antibody, Unconjugated (bs-13378R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
COLGALT1 Polyclonal AntibodyBS-13378R
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetCOLGALT1
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