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ProductsBack/Anti-Complement factor Ba [D22/3]

Anti-Complement factor Ba [D22/3]

AB02876-2.-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetCFB
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Complement factor Ba [D22/3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The mouse version of this antibody was successfully used to specifically quantitate the 34 kDa form of the Ba-fragment an ELISA was preformed on the 34kDa fragment of Ba (Oppermann and Goetze, 1994; pmid:8139584).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D22/3
  • Gene ID629
  • Target name
    CFB
  • Target description
    complement factor B
  • Target synonyms
    AHUS4, ARMD14, BF, BFD, CFAB, CFBD, FB, FBI12, GBG, H2-Bf, PBF2, complement factor B, B-factor, properdin, C3 proaccelerator, C3 proactivator, C3/C5 convertase, glycine-rich beta-glycoprotein, properdin B, properdin factor B
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP00751
  • Protein Name
    Complement factor B
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor B using anti-Factor B antibody (PB9934). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: 293T whole cell lysates, Lane 3: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor B antigen affinity purified polyclonal antibody (Catalog # PB9934) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor B at approximately 86 kDa. The expected band size for Factor B is at 86 kDa.
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