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Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.

Anti-COPE Antibody

HPA043576
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOPE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-COPE Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11316
  • Target name
    COPE
  • Target description
    coat protein complex I subunit epsilon
  • Target synonyms
    epsilon-COP, coatomer subunit epsilon, COPI coat complex subunit epsilon, coatomer epsilon subunit, coatomer protein complex subunit epsilon, epsilon coat protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14579
  • Protein Name
    Coatomer subunit epsilon
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of COPE using anti-COPE antibody (A04544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysate,Lane 2: rat small intestine tissue lysate,Lane 3: rat pancreas tissue lysate,Lane 4: mouse stomach tissue lysate,Lane 5: mouse small intestine tissue lysate,Lane 6: mouse pancreas tissue lysate,Lane 7: human MCF-7 whole Cell lysatem,Lane 8: human Hela whole Cell lysate,Lane 9: human 22RV1 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody (Catalog # A04544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
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