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Western Blot Positive WB detected in: PC-3 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate All lanes: COPE antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 29 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate All lanes: COPE antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 29 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, A375 whole cell lysate All lanes: COPE antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 29 kDa Observed band size: 35 kDa

COPE Antibody

CSB-PA03669A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCOPE
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Overview

  • Supplier
    Cusabio
  • Product Name
    COPE Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11316
  • Target name
    COPE
  • Target description
    coat protein complex I subunit epsilon
  • Target synonyms
    epsilon-COP, coatomer subunit epsilon, COPI coat complex subunit epsilon, coatomer epsilon subunit, coatomer protein complex subunit epsilon, epsilon coat protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14579
  • Protein Name
    Coatomer subunit epsilon
  • Scientific Description
    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of COPE using anti-COPE antibody (A04544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysate,Lane 2: rat small intestine tissue lysate,Lane 3: rat pancreas tissue lysate,Lane 4: mouse stomach tissue lysate,Lane 5: mouse small intestine tissue lysate,Lane 6: mouse pancreas tissue lysate,Lane 7: human MCF-7 whole Cell lysatem,Lane 8: human Hela whole Cell lysate,Lane 9: human 22RV1 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody (Catalog # A04544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
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