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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Anti-CP Antibody

HPA001834
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1356
  • Target name
    CP
  • Target description
    ceruloplasmin
  • Target synonyms
    AB073614, CP-2, ceruloplasmin, caeruloplasmin, ceruloplasmin (ferroxidase), cuproxidase ceruloplasmin, ferroxidase ceruloplasmin, glutathione peroxidase ceruloplasmin, glutathione-dependent peroxiredoxin ceruloplasmin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00450
  • Protein Name
    Ceruloplasmin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ceruloplasmin using anti-Ceruloplasmin antibody (PB9852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin antigen affinity purified polyclonal antibody (Catalog # PB9852) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ceruloplasmin at approximately 122 kDa. The expected band size for Ceruloplasmin is at 122 kDa.
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