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Anti-CPN1 Antibody

Anti-CPN1 Antibody

A101485
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-CPN1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to CPN1.
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPN1 using anti-CPN1 antibody (A05704-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPN1 antigen affinity purified polyclonal antibody (Catalog # A05704-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPN1 at approximately 52KD. The expected band size for CPN1 is at 52KD.
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Anti-CPN1 Antibody Picoband(r)A05704-2-CARRIER-FREE
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ReactivityHuman, Mouse, Rat
TargetCPN1
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TargetCPN1
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TargetCPN1
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HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CPN1 antibody [N1C3] (GTX105515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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CPN1 antibody [N1C3]GTX105515
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TargetCPN1
  • SizePrice