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Western Blot analysis of Hela cells using CPN cat Polyclonal Antibody
Western Blot analysis of Hela cells using CPN cat Polyclonal Antibody
Western Blot analysis of Hela cells using CPN cat Polyclonal Antibody

CPN1 Antibody

CSB-PA001774
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCPN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1369
  • Target name
    CPN1
  • Target description
    carboxypeptidase N subunit 1
  • Target synonyms
    CPN, SCPN, carboxypeptidase N catalytic chain, anaphylatoxin inactivator, arginine carboxypeptidase, carboxypeptidase K, carboxypeptidase N catalytic subunit, carboxypeptidase N polypeptide 1 50 kD, carboxypeptidase N small subunit, carboxypeptidase N, polypeptide 1, kininase I, kininase-1, lysine carboxypeptidase, plasma carboxypeptidase B, serum carboxypeptidase N
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15169
  • Protein Name
    Carboxypeptidase N catalytic chain
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CPN1 using anti-CPN1 antibody (A05704-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPN1 antigen affinity purified polyclonal antibody (Catalog # A05704-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPN1 at approximately 52KD. The expected band size for CPN1 is at 52KD.
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