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Immunofluorescent staining of human cell line Rh30 shows localization to plasma membrane.
Immunofluorescent staining of human cell line Rh30 shows localization to plasma membrane.
Immunofluorescent staining of human cell line Rh30 shows localization to plasma membrane.

Anti-CRLF2 Antibody

HPA075272
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCRLF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CRLF2 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64109
  • Target name
    CRLF2
  • Target description
    cytokine receptor like factor 2
  • Target synonyms
    CRL2, CRLF2Y, TSLPR, cytokine receptor-like factor 2, IL-XR, TSLP receptor, cytokine receptor CRL2 precusor, thymic stromal lymphopoietin protein receptor, thymic stromal-derived lymphopoietin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HC73
  • Protein Name
    Cytokine receptor-like factor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CRLF2 using anti-CRLF2 antibody (A04167-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CRLF2 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRLF2 antigen affinity purified polyclonal antibody (Catalog # A04167-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRLF2 at approximately 65-75KD. The expected band size for CRLF2 is at 51KD.
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