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Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.

Anti-CRP Antibody

HPA027367
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCRP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CRP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1401
  • Target name
    CRP
  • Target description
    C-reactive protein
  • Target synonyms
    PTX1, C-reactive protein, C-reactive protein, pentraxin-related, pentraxin 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02741
  • Protein Name
    C-reactive protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CRP using anti-CRP antibody (A00249-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CRP protein 10 ng, Lane 2: recombinant human CRP protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRP antigen affinity purified polyclonal antibody (Catalog # A00249-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRP at approximately 25 kDa.
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