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Anti-CRP [P20]

AB03014-1.1-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCRP
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CRP [P20]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Indirect ELISA was used for the primary screening of the hybridomas showing selective binding for human CRP. Horseradish peroxide labelled scFv P20 was used for the identification of natural CRP extracted from denatured and non-denatured blood in a western blot assay. The combination of P02 and P20 antibody was also used in the double antibody sandwich detection system consisting of P20 as the coating antibody and P02 as the labeled antibody to be used in the colloidal gold detection platform for the detection of natural CRP. These antibodies were also used in the preparation of human C-reactive protein colloidal gold test card (CN106596976).
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    P20
  • Gene ID1401
  • Target name
    CRP
  • Target description
    C-reactive protein
  • Target synonyms
    C-reactive protein; C-reactive protein, pentraxin-related; pentraxin 1; PTX1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02741
  • Protein Name
    C-reactive protein
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CRP using anti-CRP antibody (A00249-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CRP protein 10 ng, Lane 2: recombinant human CRP protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRP antigen affinity purified polyclonal antibody (Catalog # A00249-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRP at approximately 25 kDa.
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